Part:BBa_K5048043:Design

plasmid lasR

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 6545
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 351
  Illegal NgoMIV site found at 1718
  Illegal NgoMIV site found at 4765
  Illegal NgoMIV site found at 4925
  Illegal NgoMIV site found at 6513
  Illegal AgeI site found at 548
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 3844
  

Design Notes

We design this part in order to reproduce the result of the literature, except for the double terminators. we discussed with CDG China and they recommended this part because it had high efficiency.

Source

complete synthesis